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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
23/09/2016 |
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Actualizado : |
23/09/2016 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
BASILE, P.; FORMOSO, D.; TISCORNIA, G.; BLUMETTO, O. |
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Afiliación : |
PATRICIA CECILIA BASILE LORENZO, Universidad de la República (UdelaR)/ Centro Universitario Regional Tacuarembó; DANIEL FORMOSO CUNHA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OSCAR RICARDO BLUMETTO VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Radiation use efficiency on campos graslands with contrasting grzing methods. [Resumen de poster]. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
ln: Encuentro de Investigadores de la Región Noreste: Cerro Largo-Rivera-Tacuarembó, 1., 12 de agosto de 2016, Campus Interinstitucional de Tacuarembó, Tacuarembó. Libro de Resúmenes. Tacuarembó: UDELAR; INIA, 2016. |
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Páginas : |
p. 64 |
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Idioma : |
Inglés |
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Contenido : |
Introduction: In Uruguay, the Basaltic region has de highest proportion of natural grasslands of the country. In this pastures, livestock management is the main reason of degradation of natural grasslands. Today, it's possible to estimate ANPP (Aboveground Net Primary Production) using remote sensing techniques. The RUE (Radiation Use Efficiency) is the effectiveness with which fPAR (fraction of Photosyntethically Active Radiation absorbed by plants) is transformed in ANPP and is known to vary according to temperature, precipitation and species composition. Objectives; The aims of this work were: a) to calibrate RUE and b) study the temporal variability
of RUE for two contrasting grazing methods. Materials & Methods: The study was conducted on five livestock farms located in the Basaltic region. In each site, two contrasting pastures with different historical grazing management (controlled vs continuous stocking rate) were selected. Data was collected between september 2013 and february 2015. RUE coefficient was estimated following Monteith equation: RUE= ANPP / APAR and APAR= fPAR x PAR. ANPP was estimated using the technique of regrowth in three exclusion cages. Biomass was cut at 1cm in boxes 20 x 50cm with shears every 45-50 days and was dried in forced air oven at 60 ° C. fPAR
was obtained as a function of ENVI images from MODIS sensor (US Geological Survey) and PAR was estimated from agro-climatic stations of INIA. RUE data were analyzed with a oneway ANOVA and the means were compared with T test for paired samples. Results: Between grazing methods, RUE average values were statistically different (p <0.05), with controlled management reporting values above 44%. When analysing seasonal variation between grazing methods, there were no statistical differences in RUE values. Seasonal variation of RUE for each grazing methods separately, was significantly different within seasons (p <0.05). Conclusions: The RUE values obtained could be used in the estimation of a more accurately ANPP in natural grasslands of this region. MenosIntroduction: In Uruguay, the Basaltic region has de highest proportion of natural grasslands of the country. In this pastures, livestock management is the main reason of degradation of natural grasslands. Today, it's possible to estimate ANPP (Aboveground Net Primary Production) using remote sensing techniques. The RUE (Radiation Use Efficiency) is the effectiveness with which fPAR (fraction of Photosyntethically Active Radiation absorbed by plants) is transformed in ANPP and is known to vary according to temperature, precipitation and species composition. Objectives; The aims of this work were: a) to calibrate RUE and b) study the temporal variability
of RUE for two contrasting grazing methods. Materials & Methods: The study was conducted on five livestock farms located in the Basaltic region. In each site, two contrasting pastures with different historical grazing management (controlled vs continuous stocking rate) were selected. Data was collected between september 2013 and february 2015. RUE coefficient was estimated following Monteith equation: RUE= ANPP / APAR and APAR= fPAR x PAR. ANPP was estimated using the technique of regrowth in three exclusion cages. Biomass was cut at 1cm in boxes 20 x 50cm with shears every 45-50 days and was dried in forced air oven at 60 ° C. fPAR
was obtained as a function of ENVI images from MODIS sensor (US Geological Survey) and PAR was estimated from agro-climatic stations of INIA. RUE data were analyzed with a oneway ANOVA and the mea... Presentar Todo |
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Palabras claves : |
GRASSLAND PRODUCTIVITY; LIVESTOCK MANAGEMENT; PPNA. |
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Thesagro : |
PASTURAS. |
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Asunto categoría : |
P30 Ciencia del suelo y manejo del suelo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6099/1/PAGINA-64.pdf
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Marc : |
LEADER 02852naa a2200217 a 4500
001 1055722
005 2016-09-23
008 2016 bl uuuu u00u1 u #d
100 1 $aBASILE, P.
245 $aRadiation use efficiency on campos graslands with contrasting grzing methods. [Resumen de poster].$h[electronic resource]
260 $c2016
300 $ap. 64
520 $aIntroduction: In Uruguay, the Basaltic region has de highest proportion of natural grasslands of the country. In this pastures, livestock management is the main reason of degradation of natural grasslands. Today, it's possible to estimate ANPP (Aboveground Net Primary Production) using remote sensing techniques. The RUE (Radiation Use Efficiency) is the effectiveness with which fPAR (fraction of Photosyntethically Active Radiation absorbed by plants) is transformed in ANPP and is known to vary according to temperature, precipitation and species composition. Objectives; The aims of this work were: a) to calibrate RUE and b) study the temporal variability of RUE for two contrasting grazing methods. Materials & Methods: The study was conducted on five livestock farms located in the Basaltic region. In each site, two contrasting pastures with different historical grazing management (controlled vs continuous stocking rate) were selected. Data was collected between september 2013 and february 2015. RUE coefficient was estimated following Monteith equation: RUE= ANPP / APAR and APAR= fPAR x PAR. ANPP was estimated using the technique of regrowth in three exclusion cages. Biomass was cut at 1cm in boxes 20 x 50cm with shears every 45-50 days and was dried in forced air oven at 60 ° C. fPAR was obtained as a function of ENVI images from MODIS sensor (US Geological Survey) and PAR was estimated from agro-climatic stations of INIA. RUE data were analyzed with a oneway ANOVA and the means were compared with T test for paired samples. Results: Between grazing methods, RUE average values were statistically different (p <0.05), with controlled management reporting values above 44%. When analysing seasonal variation between grazing methods, there were no statistical differences in RUE values. Seasonal variation of RUE for each grazing methods separately, was significantly different within seasons (p <0.05). Conclusions: The RUE values obtained could be used in the estimation of a more accurately ANPP in natural grasslands of this region.
650 $aPASTURAS
653 $aGRASSLAND PRODUCTIVITY
653 $aLIVESTOCK MANAGEMENT
653 $aPPNA
700 1 $aFORMOSO, D.
700 1 $aTISCORNIA, G.
700 1 $aBLUMETTO, O.
773 $tln: Encuentro de Investigadores de la Región Noreste: Cerro Largo-Rivera-Tacuarembó, 1., 12 de agosto de 2016, Campus Interinstitucional de Tacuarembó, Tacuarembó. Libro de Resúmenes. Tacuarembó: UDELAR; INIA, 2016.
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Registro original : |
INIA Tacuarembó (TBO) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
26/01/2024 |
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Actualizado : |
26/01/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
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Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
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Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
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Complemento del título : |
Original full article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
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ISSN : |
1983-0246 |
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DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
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Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
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Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03124naa a2200361 a 4500
001 1064440
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
022 $a1983-0246
024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI
100 1 $aPICCOLI, R.J.
245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br --
520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved.
653 $aDiagnosis
653 $aImmunohistochemistry
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aPsittacosis
653 $aZoonosis
700 1 $aGRUCHOUSKEI, L.
700 1 $aBENINCA, A.L.V.
700 1 $aMAURENTE BERÓN, M.
700 1 $aCHENG, A.C.
700 1 $aDE ANDRADE, J.A.
700 1 $aFACCIN, M.
700 1 $aGRZEGOZEVSKI, A.P.
700 1 $aDA SILVA, G.C.R.
700 1 $aFRANÇA, J.C.
700 1 $aFERNANDES, N.L.M.
700 1 $aDE CARVALHO, A.L.
700 1 $aVIOTT, A.M.
773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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